Int J Colorectal Dis. 2005 Aug 23;:1-7 [Epub ahead of print] Links


Exacerbation of dextran sulfate sodium-induced colitis by dietary iron
supplementation: role of NF-kappaB.

Carrier JC, Aghdassi E, Jeejeebhoy K, Allard JP.

Department of Medicine, university of Toronto, Toronto, Ontario,
M5G-2C4, Canada.

BACKGROUND: In colitis, iron therapy may be given to treat anemia, but
it may also be detrimental based on our previous studies using a rat
model with colitis where iron supplementation increased disease
activity and oxidative stress. This effect was partially reduced by an
antioxidant. AIMS: The aim of this study was to further evaluate, in
rats with dextran sulfate sodium (DSS)-induced colitis, the effect of
iron on neutrophilic infiltration, cytokines and nuclear factor kappa-B
(NF-kappaB)-associated inflammation and to determine whether the
addition of vitamin E would be beneficial. METHODS: Colitis was induced
with DSS at 50 g/l in drinking water for 7 days. DSS rats were
randomized to the following: DSS, receiving a control, non-purified
diet (iron, 270 mg and DL: -alpha-tocopherol acetate, 49 mg/kg);
DSS+iron (diet+iron, 3,000 mg/kg); DSS+vitamin E (diet+DL:
-alpha-tocopherol acetate, 2,000 mg/kg); or the DSS+iron+vitamin E.
Colonic inflammation, myeloperoxidase activity (MPO), lipid peroxides
(LPO), proinflammatory cytokines [tumor necrosis factor (TNF)-alpha,
interleukin (IL)-1, IL-6] and NF-kappaB binding activity were measured.
RESULTS: The DSS+iron group showed a significant increase in
inflammatory scores, MPO, TNF-alpha, IL-1, LPO and NF-kappaB activity
compared to DSS or DSS+vitamin E. The addition of vitamin E to iron
(DSS+iron+vitamin E group) significantly reduced the inflammatory
scores, TNF-alpha and IL-6. None of the other parameters were affected.
CONCLUSION: Iron increases disease activity in colitis, and this is
associated with oxidative stress, neutrophilic infiltration, increased
cytokines and activation of NF-kappaB. This detrimental effect was
partially reduced by vitamin E.

PMID: 16133010 [PubMed - as supplied by publisher]

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Tom


Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com


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DEAD PEOPLE WALKING
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Ya good stuff! I have even heard of doctors recommending iron to there IBD
patients.
<ironjustice@aol.com> wrote in message
news:1125568127.732419.127970@f14g2000cwb.googlegroups.com..
> Int J Colorectal Dis. 2005 Aug 23;:1-7 [Epub ahead of print] Links
>
>
> Exacerbation of dextran sulfate sodium-induced colitis by dietary iron
> supplementation: role of NF-kappaB.
>
> Carrier JC, Aghdassi E, Jeejeebhoy K, Allard JP.
>
> Department of Medicine, university of Toronto, Toronto, Ontario,
> M5G-2C4, Canada.
>
> BACKGROUND: In colitis, iron therapy may be given to treat anemia, but
> it may also be detrimental based on our previous studies using a rat
> model with colitis where iron supplementation increased disease
> activity and oxidative stress. This effect was partially reduced by an
> antioxidant. AIMS: The aim of this study was to further evaluate, in
> rats with dextran sulfate sodium (DSS)-induced colitis, the effect of
> iron on neutrophilic infiltration, cytokines and nuclear factor kappa-B
> (NF-kappaB)-associated inflammation and to determine whether the
> addition of vitamin E would be beneficial. METHODS: Colitis was induced
> with DSS at 50 g/l in drinking water for 7 days. DSS rats were
> randomized to the following: DSS, receiving a control, non-purified
> diet (iron, 270 mg and DL: -alpha-tocopherol acetate, 49 mg/kg);
> DSS+iron (diet+iron, 3,000 mg/kg); DSS+vitamin E (diet+DL:
> -alpha-tocopherol acetate, 2,000 mg/kg); or the DSS+iron+vitamin E.
> Colonic inflammation, myeloperoxidase activity (MPO), lipid peroxides
> (LPO), proinflammatory cytokines [tumor necrosis factor (TNF)-alpha,
> interleukin (IL)-1, IL-6] and NF-kappaB binding activity were measured.
> RESULTS: The DSS+iron group showed a significant increase in
> inflammatory scores, MPO, TNF-alpha, IL-1, LPO and NF-kappaB activity
> compared to DSS or DSS+vitamin E. The addition of vitamin E to iron
> (DSS+iron+vitamin E group) significantly reduced the inflammatory
> scores, TNF-alpha and IL-6. None of the other parameters were affected.
> CONCLUSION: Iron increases disease activity in colitis, and this is
> associated with oxidative stress, neutrophilic infiltration, increased
> cytokines and activation of NF-kappaB. This detrimental effect was
> partially reduced by vitamin E.
>
> PMID: 16133010 [PubMed - as supplied by publisher]
>
> --------------------------------------------------------------------------
------
>
> Who loves ya.
> Tom
>
>
> Jesus Was A Vegetarian!
> http://jesuswasavegetarian.7h.com
>
>
> Man Is A Herbivore!
> http://pages.ivillage.com/ironjustice/manisaherbivore
>
>
> DEAD PEOPLE WALKING
> http://pages.ivillage.com/ironjustice/deadpeoplewalking
>

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