|
| <<snip>>
CONCLUSIONS: Non-transferrin-bound iron increases the level of intracellular
labile iron, which promotes monocyte recruitment to endothelium and may thereby
contribute to the pathogenesis of atherosclerosis.
<<snip>>
Arterioscler Thromb Vasc Biol. 2004 Oct 14 [Epub ahead of print] Links
Intracellular Labile Iron Modulates Adhesion of Human Monocytes to Human
Endothelial Cells.
Kartikasari AE, Georgiou NA, Visseren FL, van Kats-Renaud H, van Asbeck BS,
Marx JJ.
Eijkman-Winkler Center for Medical Microbiology, Infectious Diseases, and
Inflammation and Eijkman Graduate School for Immunology and Infectious
Diseases, and the Departments of Vascular Medicine and Internal Medicine,
University Medical Center Utrecht, The Netherlands.
OBJECTIVE: Elevated iron stores and high plasma iron concentration have been
linked to an increased risk of atherosclerosis. Iron may thereby affect the
interaction of monocytes to endothelium, an initial event in the formation of
atherosclerotic plaques. METHODS AND RESULTS: Addition of 10 micromol/L
non-transferrin-bound iron to the incubation medium caused a 2-fold increase in
monocyte adhesion to human umbilical vein endothelial cells (HUVECs). A
concordant increase in the expression of the following adhesion molecules was
observed: vascular cell adhesion molecule-1, intercellular adhesion molecule-1,
and endothelial selectin on HUVECs as well as very late antigen-4, and
lymphocyte function-associated antigen-1 on monocytes. The inclusion of either
deferiprone or salicylaldehyde isonicotinoylhydrazone counteracted these
effects. Intracellular iron chelation by deferoxamine was completed only after
10 hours of incubation, shown by reversal of iron-quenched intracellular
calcein signal, and concurrently the effects of iron were blunted. The
membrane-impermeable chelator, diethylenetriamine pentaaceticacid, failed to
negate iron effects, even after 48 hours of treatment. Furthermore, only
membrane-permeable superoxide or hydroxyl radical scavengers were capable of
preventing HUVEC activation by iron. CONCLUSIONS: Non-transferrin-bound iron
increases the level of intracellular labile iron, which promotes monocyte
recruitment to endothelium and may thereby contribute to the pathogenesis of
atherosclerosis. Iron-induced adhesion molecule expression was observed, and
this event may involve the production of oxygen radicals.
PMID: 15486315 [PubMed - as supplied by publisher]
--------------------------------------------------------------------------
------
Who loves ya.
Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking
|
|