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Home > Archive > Medicine laboratory > September 2006 > Candida and iron
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| ironjustice@aol.com 2006-09-09, 2:26 am |
| Unexpected link between iron and drug resistance of Candida: iron
depletion enhances membrane fluidity and drug diffusion leading to drug
susceptible cells.
Prasad T, Chandra A, Mukhopadhyay CK, Prasad R
Antimicrob Agents Chemother. 2006 Sep 5;
In this study, we show that the iron depletion in C. albicans using
bathophenanthrolene disulphonic acid (BPS) and ferrozine as chelators
enhanced its sensitivity to several drugs including the most common
antifungal, fluconazole (FLC). Several other species of Candida also
displayed increased sensitivity to FLC due to iron restriction. The
iron uptake mutants namely Deltaftr1 and Deltaftr2, as well as the
copper transporter mutant Deltaccc2, that affects the high affinity
iron uptake in Candida, showed an increased sensitivity to FLC as
compared to the wild type. The effect of iron depletion on drug
sensitivity appeared to be independent of efflux pump proteins, Cdr1p
and Cdr2p. We found that iron deprivation lead to lowering of membrane
ergosterol by 15-30 %. Subsequently, fluorescence polarization
measurements also revealed that iron-restricted Candida cells displayed
29-40 % increase in membrane fluidity resulting in enhanced passive
diffusion of the drugs. Northern blots revealed that ERG11 gene was
considerably down-regulated in iron-deprived cells which might account
for the lowered ergosterol content. Our results show a close
relationship between cellular iron and drug susceptibilities of C.
albicans. Considering that MDR is a manifestation of multifactorial
phenomenon, the influence of cellular iron on drug susceptibilities of
Candida suggests iron as yet another novel determinant of multi-drug
resistance.
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk
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| Kill filed....
<ironjustice@aol.com> wrote in message
news:1157770558.628045.19850@e3g2000cwe.googlegroups.com...
> Unexpected link between iron and drug resistance of Candida: iron
> depletion enhances membrane fluidity and drug diffusion leading to drug
>
> susceptible cells.
> Prasad T, Chandra A, Mukhopadhyay CK, Prasad R
> Antimicrob Agents Chemother. 2006 Sep 5;
>
> In this study, we show that the iron depletion in C. albicans using
> bathophenanthrolene disulphonic acid (BPS) and ferrozine as chelators
> enhanced its sensitivity to several drugs including the most common
> antifungal, fluconazole (FLC). Several other species of Candida also
> displayed increased sensitivity to FLC due to iron restriction. The
> iron uptake mutants namely Deltaftr1 and Deltaftr2, as well as the
> copper transporter mutant Deltaccc2, that affects the high affinity
> iron uptake in Candida, showed an increased sensitivity to FLC as
> compared to the wild type. The effect of iron depletion on drug
> sensitivity appeared to be independent of efflux pump proteins, Cdr1p
> and Cdr2p. We found that iron deprivation lead to lowering of membrane
> ergosterol by 15-30 %. Subsequently, fluorescence polarization
> measurements also revealed that iron-restricted Candida cells displayed
>
> 29-40 % increase in membrane fluidity resulting in enhanced passive
> diffusion of the drugs. Northern blots revealed that ERG11 gene was
> considerably down-regulated in iron-deprived cells which might account
> for the lowered ergosterol content. Our results show a close
> relationship between cellular iron and drug susceptibilities of C.
> albicans. Considering that MDR is a manifestation of multifactorial
> phenomenon, the influence of cellular iron on drug susceptibilities of
> Candida suggests iron as yet another novel determinant of multi-drug
> resistance.
>
>
> Who loves ya.
> Tom
>
>
> Jesus Was A Vegetarian!
> http://jesuswasavegetarian.7h.com
>
>
> Man Is A Herbivore!
> http://tinyurl.com/a3cc3
>
>
> DEAD PEOPLE WALKING
> http://tinyurl.com/zk9fk
>
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| Ad hominem 2006-09-13, 8:25 am |
| This cock wouldn't even care if you killfiled him. He another
delusional crackpot that crossposts irrelavent and inaccurate crap.
Buhda wrote:[vbcol=seagreen]
> Kill filed....
> <ironjustice@aol.com> wrote in message
> news:1157770558.628045.19850@e3g2000cwe.googlegroups.com...
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