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Home > Archive > Medicine laboratory > March 2006 > post vas sperm evaluation
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post vas sperm evaluation
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| Candice Ford 2006-03-22, 3:40 pm |
| For post vasectomy qualitative semen evaluation (presence or absence of
sperm only): Does anyone centrifuge semen before examining? If so, how
many rpms? How long? Is there a standardized procedure? Any sites with
helpful info and sources?
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| Jflagg 2006-03-22, 3:40 pm |
| CAP requires that all post vas specimens and those fertility specimens
showing azoospermia or oligozoospermia need to be concentrated. Our
procedure is to let the semen liquify and then centrifuge like a urine
sample. (5 min at 1500rpm), then examine the button for sperm.
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| harrison6723@rogers.com 2006-03-22, 3:40 pm |
| Here you go...
Principle:
To evaluate a seminal fluid for absence of spermatozoa following a
vasectomy
Patient and Specimen Requirements:
Conditions for Patient Preparation
1=2E Patient will follow the physician's orders for the required
time interval between the vasectomy procedure and seminal analysis
testing - usually 2 months.
2=2E Patient will collect the specimen by masturbation, not by
coitus interuptus.
3=2E Patient will collect the specimen in a clean, dry, sterile
container that is supplied by the lab, or the physician's office.
Specimen Type
=B7 Semen sample
Material Requirements and Preparation:
Urinalysis centrifuge tube
Glass slide
Glass coverslip
Procedure:
1=2E Transfer specimen to a urinalysis centrifuge tube.
2=2E Centrifuge at 2000 rpm for 5 minutes.
3=2E Aspirate supernatant with pipette and deposit back into original
container.
4=2E Place one drop of sediment onto glass slide. Coverslip with a 22 x
22 glass coverslip.
5=2E Scan entire preparation using x40 objective for spermatozoa.
Reference Intervals and Interpretation:
Choose one of the queries listed as required at each of the result
entry fields.
Sperm: none seen/present
Quantity: rare <5/hpf
few 6-10/hpf
moderate 11-20/hpf
many >20/hpf
Motility: non-motile/motile
References:
Cannon,D. Examination of Seminal Fluid.
Knight, Kjeldsberg. Blody Fluids 2nd Edition.
Consultation with SWCHSC Clinical Pathology Department
Adelman, M, Cahill,E. Atlas of Sperm Morphology. ASCP. 1989.
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