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Home > Archive > Medicine laboratory > October 2006 > how can i isolate Rotavirus??
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how can i isolate Rotavirus??
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| Raja' MT 2006-10-03, 4:28 pm |
| hellooo everybody..
i'm a med. tech. intern,, i'm training now in virology lab
i need to know in which cell line can we isolate Rotavirus??
cuz in this lab, they use the latex agglutination method to detect
rotavirus infections,
it's really worthless, i guess!!
cuz i have to repeat the test 3 times with 3 different kits, to make
sure that a positive one it's a real positve( i've never heard about
such a protocol). all these kits have a variable % of sensitivity, & i
really prefer the isolation method!!
thanx
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| JEDilworth 2006-10-04, 2:32 am |
| http://www.remelinc.com/products/cl...2/Rotavirus.cfm
We use the Remel SAS Rotavirus test. It takes 15 minutes and works very
well. Our docs can't wait that long for results.
The latex agglutination tests are not as good as the EIA tests. You should
really look into those. I haven't done latex testing for Rotavirus in at
least 15 years.
If you're a MT intern, you're pretty much bound to do things by whatever
rules the lab you're working for uses. You can't just go off on your own and
decide to grow rotavirus as a methodology. Student MT's are pretty much at
the bottom of the pecking order in laboratories.
I would think that all the bacteria in stools would contaminate your cell
lines with bacterial overgrowth. I can't tell you the last time I've seen an
order for viral culture on a stool.
Judy Dilworth, M.T. (ASCP)
Microbiology
"Raja' MT" <med.tech.alahmadi@gmail.com> wrote in message
news:1159896177.622667.115370@i3g2000cwc.googlegroups.com...
> hellooo everybody..
>
> i'm a med. tech. intern,, i'm training now in virology lab
>
> i need to know in which cell line can we isolate Rotavirus??
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| Raja' MT 2006-10-05, 8:30 am |
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15 YEARS!!!
Woooo,, that's a really long time! I can believe that we still depend
on it in diagnosis.
You're right about us " intern students", but here's the good
news..
We can with the help from our Dr's in our college do our own small
researches during the internship year. It has nothing to do with our
bachelor degree project (which actually what we did last year). The
main aim is to help the labs that we're training in to improve their
work or quality. In the end of every year, we have a students meeting
organized by our college where we present our works and projects. The
college invites many heads of lab from different hospitals & medical
centers in the city to that meeting.
& that's what I'm trying to do,, I want to prove to my lab that
this latex method it's not useful & they have to find another way.
Getting a 1 ml of cell line suspension & then subculture it is much
easiest for me than ordering a kit. But I will use your idea to include
it in my thesis.
Did you mean that I can't culture a stool specimen??
If so,, how can I process such a specimen for viral studies?
Thank you for replying
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| JEDilworth 2006-10-05, 4:27 pm |
| I don't work in Virology so I don't know how stools are processed. I
shouldn't have spoken out of turn.
Any Virology people in clinical labs out there? Please help!
I don't know where your lab is located but they really need to review
current methodologies.
Judy Dilworth, M.T. (ASCP)
Microbiology
"Raja' MT" <med.tech.alahmadi@gmail.com> wrote in message
news:1160046747.635335.107000@b28g2000cwb.googlegroups.com...
>
> 15 YEARS!!!
> Woooo,, that's a really long time! I can believe that we still depend
> on it in diagnosis.
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