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Home > Archive > Medicine laboratory > June 2005 > Hematology cap checklist question, Retic's, help?
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Hematology cap checklist question, Retic's, help?
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| Katra 2005-05-18, 11:23 am |
| HEM.35150 deals with interferance in automated reticulocyte counts.
I've done some google searches and there is pretty much no information
available on the Coulter VCS flow cytometer technology.
We only started doing automated retic's earlier this year, and now we
need to deal with that cap question. ;-)
I understand how VCS works pretty much, and Coulter uses a methelyne
blue stain as part of the retic counting in the laser flow cytometer,
but I am not sure what, if anything, will interfere with that specific
method!
And I'd need documentation in writing for the CAP. I need to fix our
protcal for this question to be in compliance.
I've contacted Coulter, but they have been EVER so slow in responding!
Even our HAS has not yet responded to our inquiries. :-( It's beginning
to tick me off.
Does anyone know where I can find the information I need?????
Thanks!
--
K.
Sprout the MungBean to reply
"I don't like to commit myself about heaven and hell‹you
see, I have friends in both places." --Mark Twain
| |
| Robert 2005-05-18, 11:23 am |
|
"Katra" <KatraMungBean@Centurytel.net> wrote in message
news:KatraMungBean-E1C2A7.07232114052005@corp.supernews.com...
> HEM.35150 deals with interferance in automated reticulocyte counts.
> I've done some google searches and there is pretty much no information
> available on the Coulter VCS flow cytometer technology.
>
> We only started doing automated retic's earlier this year, and now we
> need to deal with that cap question. ;-)
>
> I understand how VCS works pretty much, and Coulter uses a methelyne
> blue stain as part of the retic counting in the laser flow cytometer,
> but I am not sure what, if anything, will interfere with that specific
> method!
>
> And I'd need documentation in writing for the CAP. I need to fix our
> protcal for this question to be in compliance.
>
> I've contacted Coulter, but they have been EVER so slow in responding!
> Even our HAS has not yet responded to our inquiries. :-( It's beginning
> to tick me off.
>
> Does anyone know where I can find the information I need?????
>
> Thanks!
> --
> K.
I wrote a series of cont 'ed on pseudoreticulocytosis and interference with
our CD4000. Unfortunately I am not familiar with the technology you use.
Keep in mind that MB is not specific for reticulocytes. HB bodies, Heinz
bodies, basophilic stippling all stain with MB and are not retics.
We perform smear reviews for any auto retic greater than 8%. We look for red
cell inclusions on the above. It is also a good idea to check for
hyposplenism and Keratocytes (Blister cell, eccentrocytes, helmet cells,
bite cells, and irregularly contracted cells sometimes called spherocytes.
Those are associated with heinz body anemias. Heinz bodies are not seen with
wrights stain but do stain with MB and are seen with flow instruments with
laser.
Malarial parasites I don't know with your instrument? Vivax infects the
retics so no false increase there but it does with falciparum as it infects
both young and old cells.
Any smear not showing consistent polychromasia or increased red cell
inclusions are performed with a manual retic.
Sorry as I don't have any Coulter stuff.
Is it Coulter that offers the CHr retic hemoglobin content?
>
> Sprout the MungBean to reply
>
> "I don't like to commit myself about heaven and hell > see, I have friends
in both places." --Mark Twain
| |
| Katra 2005-05-18, 11:23 am |
| In article <mZWdna8B7N1r3hvfRVn-sw@got.net>,
"Robert" <Robertitsme@hotmail.com> wrote:
> "Katra" <KatraMungBean@Centurytel.net> wrote in message
> news:KatraMungBean-E1C2A7.07232114052005@corp.supernews.com...
>
> I wrote a series of cont 'ed on pseudoreticulocytosis and interference with
> our CD4000. Unfortunately I am not familiar with the technology you use.
> Keep in mind that MB is not specific for reticulocytes. HB bodies, Heinz
> bodies, basophilic stippling all stain with MB and are not retics.
> We perform smear reviews for any auto retic greater than 8%. We look for red
> cell inclusions on the above. It is also a good idea to check for
> hyposplenism and Keratocytes (Blister cell, eccentrocytes, helmet cells,
> bite cells, and irregularly contracted cells sometimes called spherocytes.
> Those are associated with heinz body anemias. Heinz bodies are not seen with
> wrights stain but do stain with MB and are seen with flow instruments with
> laser.
> Malarial parasites I don't know with your instrument? Vivax infects the
> retics so no false increase there but it does with falciparum as it infects
> both young and old cells.
> Any smear not showing consistent polychromasia or increased red cell
> inclusions are performed with a manual retic.
> Sorry as I don't have any Coulter stuff.
> Is it Coulter that offers the CHr retic hemoglobin content?
Coulter offers an immature reticulocyte fraction.
I finally got ahold of our HAS, and she is going to fax me the
information that I needed.
Thanks! :-)
--
K.
Sprout the MungBean to reply
"I don't like to commit myself about heaven and hell‹you
see, I have friends in both places." --Mark Twain
| |
| Robert 2005-05-18, 11:23 am |
|
"Katra" <KatraMungBean@Centurytel.net> wrote in message
news:KatraMungBean-E1C2A7.07232114052005@corp.supernews.com...
> HEM.35150 deals with interferance in automated reticulocyte counts.
> I've done some google searches and there is pretty much no information
> available on the Coulter VCS flow cytometer technology.
>
> We only started doing automated retic's earlier this year, and now we
> need to deal with that cap question. ;-)
>
> I understand how VCS works pretty much, and Coulter uses a methelyne
> blue stain as part of the retic counting in the laser flow cytometer,
> but I am not sure what, if anything, will interfere with that specific
> method!
>
> And I'd need documentation in writing for the CAP. I need to fix our
> protcal for this question to be in compliance.
>
> I've contacted Coulter, but they have been EVER so slow in responding!
> Even our HAS has not yet responded to our inquiries. :-( It's beginning
> to tick me off.
>
> Does anyone know where I can find the information I need?????
>
> Thanks!
> --
> K.
I wrote a series of cont 'ed on pseudoreticulocytosis and interference with
our CD4000. Unfortunately I am not familiar with the technology you use.
Keep in mind that MB is not specific for reticulocytes. HB bodies, Heinz
bodies, basophilic stippling all stain with MB and are not retics.
We perform smear reviews for any auto retic greater than 8%. We look for red
cell inclusions on the above. It is also a good idea to check for
hyposplenism and Keratocytes (Blister cell, eccentrocytes, helmet cells,
bite cells, and irregularly contracted cells sometimes called spherocytes.
Those are associated with heinz body anemias. Heinz bodies are not seen with
wrights stain but do stain with MB and are seen with flow instruments with
laser.
Malarial parasites I don't know with your instrument? Vivax infects the
retics so no false increase there but it does with falciparum as it infects
both young and old cells.
Any smear not showing consistent polychromasia or increased red cell
inclusions are performed with a manual retic.
Sorry as I don't have any Coulter stuff.
Is it Coulter that offers the CHr retic hemoglobin content?
>
> Sprout the MungBean to reply
>
> "I don't like to commit myself about heaven and hell > see, I have friends
in both places." --Mark Twain
| |
| kuhnfucius 2005-06-21, 10:54 pm |
| I'll have to see how I handle this check point. I have found many times in
the passed that going to B/C for help is generally useless, as there never
seems to be much theoretical help ( field service excellent though). The
question you touched on in your post was a concern of mind about two years
back and I just don't remember how I wrote the proceedure (lot of help from
the spell "chequer".)
"Katra" <KatraMungBean@Centurytel.net> wrote in message
news:KatraMungBean-E1C2A7.07232114052005@corp.supernews.com...
> HEM.35150 deals with interferance in automated reticulocyte counts.
> I've done some google searches and there is pretty much no information
> available on the Coulter VCS flow cytometer technology.
>
> We only started doing automated retic's earlier this year, and now we
> need to deal with that cap question. ;-)
>
> I understand how VCS works pretty much, and Coulter uses a methelyne
> blue stain as part of the retic counting in the laser flow cytometer,
> but I am not sure what, if anything, will interfere with that specific
> method!
>
> And I'd need documentation in writing for the CAP. I need to fix our
> protcal for this question to be in compliance.
>
> I've contacted Coulter, but they have been EVER so slow in responding!
> Even our HAS has not yet responded to our inquiries. :-( It's beginning
> to tick me off.
>
> Does anyone know where I can find the information I need?????
>
> Thanks!
> --
> K.
>
> Sprout the MungBean to reply
>
> "I don't like to commit myself about heaven and hell > see, I have friends
> in both places." --Mark Twain
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