Psoriasis support - Please translate scientistspeak

Author

Please translate scientistspeak

manfred95@lycos.com

2006-04-12, 6:17 pm

Can anyone translate this into plain english for the science impaired?
Thank you.

Catalase regulates cell growth in HL60 human promyelocytic cells:
evidence for growth regulation by H(2)O(2).

Hachiya M, Akashi M.

Department of Radiation Emergency Medicine, The Research Center for
Radiation Emergency Medicine, National Institute of Radiological
Sciences, Chiba-city, Chiba 263-8555, Japan.

Reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2))
are generated constitutively in mammalian cells. Because of its
relatively long life and high permeability across membranes, H(2)O(2)
is thought to be an important second messenger. Generation of H(2)O(2)
is increased in response to external insults, including radiation.
Catalase is located at the peroxisome and scavenges H(2)O(2). In this
study, we investigated the role of catalase in cell growth using the
H(2)O(2)-resistant variant HP100-1 of human promyelocytic HL60 cells.
HP100-1 cells had an almost 10-fold higher activity of catalase than
HL60 cells without differences in levels of glutathione peroxidase,
manganese superoxide dismutase (MnSOD), and copper-zinc SOD (CuZnSOD).
HP100-1 cells had higher proliferative activity than HL60 cells.
Treatment with catalase or the introduction of catalase cDNA into HL60
cells stimulated cell growth. Exposure of HP100-1 cells to a catalase
inhibitor resulted in suppression of cell growth with concomitant
increased levels of intracellular H(2)O(2). Moreover, exogenously added
H(2)O(2) or depletion of glutathione suppressed cell growth in HL60
cells. Extracellular signal regulated kinase 1/2 (ERK1/2) was
constitutively phosphorylated in HP100-1 cells but not in HL60 cells.
Inhibition of the ERK1/2 pathway suppressed the growth of HP100-1
cells, but inhibition of p38 mitogen-activated protein kinase (p38MAPK)
did not affect growth. Moreover, inhibition of catalase blocked the
phosphorylation of ERK1/2 but not of p38MAPK in HP100-1 cells. Thus our
results suggest that catalase activates the growth of HL60 cells
through dismutation of H(2)O(2), leading to activation of the ERK1/2
pathway; H(2)O(2) is an important regulator of growth in HL60 cells.

PMID: 15733034 [PubMed - indexed for MEDLINE]

randall

2006-04-13, 1:18 am

manfred95@lycos.com wrote:
> Can anyone translate this into plain english for the science impaired?
> Thank you.

How many times have i said to use the medical dictionary?

http://www.online-medical-dictionary.org/omd.asp?q=

I'll do a few and you can then tell us whats going on.

Catalase

An oxidoreductase that catalyzes the conversion of hydrogen peroxide to
water and oxygen. It is present in many animal cells. A deficiency of
this enzyme results in ACATALASIA.

Promyelocytes
Leukocytes with abundant granules in the cytoplasm. They are divided
into three groups: NEUTROPHILS; EOSINOPHILS; and BASOPHILS.

Reactive Oxygen Species
Molecules or ions formed by the incomplete one-electron reduction of
oxygen. These reactive oxygen intermediates include SINGLET OXYGEN,
SUPEROXIDES, PEROXIDES, HYDROXYL RADICAL and HYPOCHLOROUS ACID. They
contribute to the microbicidal activity of PHAGOCYTES, regulation of
signal transduction and gene expression, and the oxidative damage to
NUCLEIC ACIDS, PROTEINS, and LIPIDS.

>
> Catalase regulates cell growth in HL60 human promyelocytic cells:

Cells
Minute protoplasmic masses that make up organized tissue, consisting of
a nucleus which is surrounded by protoplasm which contains the various
organelles and is enclosed in the cell or plasma membrane. Cells are
the fundamental, structural, and functional units of living organisms.
(Dorland, 28th ed)

> evidence for growth regulation by H(2)O(2).

While your presently looking at H2O2, i've been reporting on AA
(arachidonic acid)
and am just as concerned with any info on that pathway,
http://www.ncbi.nlm.nih.gov/entrez/...st_uids=9668117

So lets look at your hl60 cancer cells with AA,
http://www.ncbi.nlm.nih.gov/entrez/...rachidonic+acid

And compare it with a check of psoriasis and AA,
http://www.ncbi.nlm.nih.gov/entrez/...rachidonic+acid

So? Lets do h2O2 and P,
http://www.ncbi.nlm.nih.gov/entrez/...=psoriasis+h2o2

By shear weigh alone, while h2O2 may be a factor, scientist are looking
at AA a lot
closer.

Not that it isn't on your radars now.

>
> Hachiya M, Akashi M.
>
> Department of Radiation Emergency Medicine, The Research Center for
> Radiation Emergency Medicine, National Institute of Radiological
> Sciences, Chiba-city, Chiba 263-8555, Japan.
>
> Reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2))
> are generated constitutively in mammalian cells. Because of its
> relatively long life and high permeability across membranes, H(2)O(2)
> is thought to be an important second messenger. Generation of H(2)O(2)
> is increased in response to external insults, including radiation.

IOWs you can lay around in the sun and produce hydrogen peroxide.

Ask your self this question. Is psoriasis compensatory the same way
h2o2 is? And if so, whats the stressor?

Mikhails group B strep perhaps?

> Catalase is located at the peroxisome and scavenges H(2)O(2). In this
> study, we investigated the role of catalase in cell growth using the
> H(2)O(2)-resistant variant HP100-1 of human promyelocytic HL60 cells.

Try this one on for size,
http://www.ncbi.nlm.nih.gov/entrez/...5&dopt=Abstract

For vk3,
http://www.greatvistachemicals.com/...vitamin-k3.html

Eat your veggies to make h2o2 the natural way? Huh!

> HP100-1 cells had an almost 10-fold higher activity of catalase than
> HL60 cells without differences in levels of glutathione peroxidase,

Glutathione Peroxidase
An enzyme catalyzing the oxidation of 2 moles of glutathione in the
presence of hydrogen peroxide to yield oxidized glutathione and water.

> manganese superoxide dismutase (MnSOD),

Manganese Superoxide Dismutase
An oxidoreductase that catalyzes the reaction between superoxide anions
and hydrogen to yield molecular oxygen and hydrogen peroxide. The
enzyme protects the cell against dangerous levels of superoxide.

> and copper-zinc SOD (CuZnSOD).

http://www.altonweb.com/cs/downsynd...m?page=sod.html

> HP100-1 cells had higher proliferative activity than HL60 cells.
> Treatment with catalase or the introduction of catalase cDNA into HL60
> cells stimulated cell growth. Exposure of HP100-1 cells to a catalase
> inhibitor resulted in suppression of cell growth with concomitant
> increased levels of intracellular H(2)O(2). Moreover, exogenously added
> H(2)O(2) or depletion of glutathione suppressed cell growth in HL60
> cells. Extracellular signal regulated kinase 1/2 (ERK1/2) was
> constitutively phosphorylated in HP100-1 cells but not in HL60 cells.
> Inhibition of the ERK1/2 pathway suppressed the growth of HP100-1
> cells, but inhibition of p38 mitogen-activated protein kinase (p38MAPK)
> did not affect growth. Moreover, inhibition of catalase blocked the
> phosphorylation of ERK1/2 but not of p38MAPK in HP100-1 cells. Thus our
> results suggest that catalase activates the growth of HL60 cells
> through dismutation of H(2)O(2), leading to activation of the ERK1/2
> pathway; H(2)O(2) is an important regulator of growth in HL60 cells.
>
> PMID: 15733034 [PubMed - indexed for MEDLINE]

Eat you veggies and don't run out of catalase? Keep your SOD, gpx
levels high and wear clean undies.

But i've sorta felt that folks with P have a slight margin of error
weighing in
their favor when it comes to cancer. Th1 skew (making P) being the
less evil
over a Th2 skew that may lead to cancer conditions.

Well, you should be able to figure out the rest.

randall