| Jan van Roijen 2005-05-03, 11:47 am |
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Send an Email for free membership
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3 May 2005
Editorship : j.van.roijen@chello.nl
Outgoing mail scanned by Norton AV
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From: DEnlander@aol.com
Ciguatera Neurotoxin Testing Protocol
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Dr Hokama have resumed the Ciguatera neurotoxin Testing
Protocol in CFS after the terrible fire in his lab. Patients in the
New York area wishing to have this test should contact us for
further details
Derek Enlander MD
1: Hokama Y, Uto GA, Palafox NA, Enlander D, Jordan E,
Cocchetto A. - Chronic phase lipids in sera of chronic fatigue
syndrome (CFS), chronic ciguatera fish poisoning (CCFP),
hepatitis B, and cancer with antigenic epitope resembling
ciguatoxin, as assessed with MAb-CTX. -J Clin Lab Anal.
2003;17(4):132-9. - PMID: 12784262 [PubMed - index
MEDLINE]
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As a reminder I have added the abstract of the study below.
~jvr
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Chronic phase lipids in sera of chronic fatigue syndrome
(CFS), chronic ciguatera fish poisoning (CCFP), hepatitis
B, and cancer with antigenic epitope resembling ciguatoxin,
as assessed with MAb-CTX.
Journal: J Clin Lab Anal. 2003;17(4):132-9.
Authors: Hokama Y, Uto GA, Palafox NA, Enlander D, Jordan E,
Cocchetto A.
Affiliation: Department of Pathology, university of
Hawaii-Manoa, Honolulu, Hawaii.
NLM Citation: PMID: 12784262
Clinical reports and descriptions of chronic fatigue syndrome
(CFS) and chronic ciguatera fish poisoning (CCFP) show great
similarities in clinical symptomology. These similarities in the
literature suggested the exploration of lipids in sera of CFS,
CCFP, and other diseases with the membrane immunobead
assay (MIA), which is typically used for screening ciguateric
ocean fish.
Sera from patients with other diseases, including hepatitis B,
cancer, and diabetes, were included to assess the degree of
specificity involved. Sera were treated with acetone in a ratio of
1 part serum to 4 parts acetone. The suspension was
centrifuged, and the acetone layer was evaporated. The residue
was weighed and redissolved in 1.0 mL methanol and tested by
the MIA, undiluted and titered to 1:160.
The undiluted acetone fraction of the 37 normal showed +/-
activity to +activity with 16 no titer, 15 with 1:5 titer and two with
1:10 titer, and four with >/=1:40 titers. One hundred fifteen CFS
sera showed 1 with 1+ and 114 with 2+ activity in the undiluted
samples, 1 with 1:10 titer, 3 with 1:20 titer, 31 with 1:40 titer, 50
with 1:80 titer, and 30 with 160 titer. Thus 95.6% of the samples
had >/=1:40 titer. Eight hepatitis B sera samples had >/=1:40
titers. Four CCFP samples had >/=1:40 titers. Three of 16
cancer samples had 1:40 titer. These data are summarized in
Fig. 1. As shown in Table 1, a significant increase (P<0.001) in
the chronic phase lipids (CPLs) was shown relative to the
normal group.
A preliminary chemical study in C18 octadecylsilyl columns
showed all fractions (100% chloroform, 9:1 chloroform :
methanol, 1:1 chloroform : methanol, and 100% methanol) to
contain lipids reactive to MAb-CTX with different intensities.
Prostaglandins were shown in 100% methanol fraction.
Competitive MIA with crude fish ciguatoxin and CFS with
synthetic JKLM ciguatoxin epitope suggested similarities in
structure with ciguatoxin. This was compatible with the
neuroblastoma assay demonstrated in the C(18) column
fractions 9:1 and 1:1, chloroform : methanol solvents.
Copyright 2003 John Wiley & Sons, Ltd.
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