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Home > Archive > Diabetes > April 2006 > Chloroquine inhibits fungal infection by limiting iron
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Chloroquine inhibits fungal infection by limiting iron
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| ironjustice@aol.com 2006-04-21, 10:53 am |
| Chloroquine Inhibits Paracoccidioides brasiliensis Survival within
Human Monocytes by Limiting the Availability of Intracellular Iron.
Dias-Melicio LA, Moreira AP, Calvi SA, Soares AM
Microbiol Immunol. 2006; 50(4): 307-14
The mechanisms used by Paracoccidioides brasiliensis(Pb 18) to survive
into monocytes are not clear. Cellular iron metabolism is of critical
importance to the growth of several intracellular pathogens, including
P. brasiliensis, whose capacity to multiply in mononuclear phagocytes
is dependent on the availability of intracellular iron. Chloroquine, by
virtue of its basic properties, has been shown to prevent release of
iron from holotransferrin by raising endocytic and lysosomal pH, and
thereby interfering with normal iron metabolism. Then, in view of this,
we have studied the effects of CHLOR on P. brasiliensis multiplication
in human monocytes and its effect on the murine paracoccidioidomycosis.
CHLOR induced human monocytes to kill P. brasiliensis. The effect of
CHLOR was reversed by FeNTA, an iron compound that is soluble at
neutral to alkaline pH, but not by holotransferrin, which releases iron
only in an acidic environment. CHLOR treatment of Pb 18-infected BALB/c
mice significantly reduced the viable fungi recovery from lungs, during
three different periods of evaluation, in a dose-dependent manner. This
study demonstrates that iron is of critical importance to the survival
of P. brasiliensis yeasts within human monocytes and the CHLOR
treatment in vitro induces Pb 18 yeast-killing by monocytes by
restricting the availability of intracellular iron. Besides, the CHLOR
treatment in vivo significantly reduces the number of organisms in the
lungs of Pb-infected mice protecting them from several infections.
Thus, CHLOR was effective in the treatment of murine
paracoccidioidomycosis, suggesting the potential use of this drug in
patients' treatment.
http://www.doctorfungus.org/mycoses...idiomycosis.htm
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING
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| Steve O 2006-04-22, 10:52 am |
| have you ever read Udo Erasmus?
http://www.udoerasmus.com/firstscreen.htm
--
Steve O
A stupid man's report of what a clever man says can never be accurate,
because he unconsciously translates what he hears into something he can
understand.
-- Bertrand Russell
<ironjustice@aol.com> wrote in message
news:1145630201.878744.40490@j33g2000cwa.googlegroups.com...
> Chloroquine Inhibits Paracoccidioides brasiliensis Survival within
> Human Monocytes by Limiting the Availability of Intracellular Iron.
> Dias-Melicio LA, Moreira AP, Calvi SA, Soares AM
> Microbiol Immunol. 2006; 50(4): 307-14
>
> The mechanisms used by Paracoccidioides brasiliensis(Pb 18) to survive
> into monocytes are not clear. Cellular iron metabolism is of critical
> importance to the growth of several intracellular pathogens, including
> P. brasiliensis, whose capacity to multiply in mononuclear phagocytes
> is dependent on the availability of intracellular iron. Chloroquine, by
> virtue of its basic properties, has been shown to prevent release of
> iron from holotransferrin by raising endocytic and lysosomal pH, and
> thereby interfering with normal iron metabolism. Then, in view of this,
> we have studied the effects of CHLOR on P. brasiliensis multiplication
> in human monocytes and its effect on the murine paracoccidioidomycosis.
> CHLOR induced human monocytes to kill P. brasiliensis. The effect of
> CHLOR was reversed by FeNTA, an iron compound that is soluble at
> neutral to alkaline pH, but not by holotransferrin, which releases iron
> only in an acidic environment. CHLOR treatment of Pb 18-infected BALB/c
> mice significantly reduced the viable fungi recovery from lungs, during
> three different periods of evaluation, in a dose-dependent manner. This
> study demonstrates that iron is of critical importance to the survival
> of P. brasiliensis yeasts within human monocytes and the CHLOR
> treatment in vitro induces Pb 18 yeast-killing by monocytes by
> restricting the availability of intracellular iron. Besides, the CHLOR
> treatment in vivo significantly reduces the number of organisms in the
> lungs of Pb-infected mice protecting them from several infections.
> Thus, CHLOR was effective in the treatment of murine
> paracoccidioidomycosis, suggesting the potential use of this drug in
> patients' treatment.
>
>
> http://www.doctorfungus.org/mycoses...idiomycosis.htm
>
> Who loves ya.
> Tom
>
>
> Jesus Was A Vegetarian!
> http://jesuswasavegetarian.7h.com
>
>
> Man Is A Herbivore!
> http://pages.ivillage.com/ironjustice/manisaherbivore
>
>
> DEAD PEOPLE WALKING
> http://pages.ivillage.com/ironjustice/deadpeoplewalking
>
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