| Author |
Metal chelator 1,000-fold greater killing / infection
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| ironjustice@aol.com 2006-03-22, 10:47 am |
| Chelator-Induced Dispersal and Killing of Pseudomonas aeruginosa Cells
in a Biofilm.
Banin E, Brady KM, Greenberg EP
Appl Environ Microbiol. 2006 Mar ; 72(3): 2064-9
Biofilms consist of groups of bacteria attached to surfaces and encased
in a hydrated polymeric matrix. Bacteria in biofilms are more resistant
to the immune system and to antibiotics than their free-living
planktonic counterparts. Thus, biofilm-related infections are
persistent and often show recurrent symptoms. The metal chelator EDTA
is known to have activity against biofilms of gram-positive bacteria
such as Staphylococcus aureus. EDTA can also kill planktonic cells of
Proteobacteria like Pseudomonas aeruginosa. In this study we
demonstrate that EDTA is a potent P. aeruginosa biofilm disrupter. In
Tris buffer, EDTA treatment of P. aeruginosa biofilms results in
1,000-fold greater killing than treatment with the P. aeruginosa
antibiotic gentamicin. Furthermore, a combination of EDTA and
gentamicin results in complete killing of biofilm cells. P. aeruginosa
biofilms can form structured mushroom-like entities when grown under
flow on a glass surface. Time lapse confocal scanning laser microscopy
shows that EDTA causes a dispersal of P. aeruginosa cells from biofilms
and killing of biofilm cells within the mushroom-like structures. An
examination of the influence of several divalent cations on the
antibiofilm activity of EDTA indicates that magnesium, calcium, and
iron protect P. aeruginosa biofilms against EDTA treatment. Our results
are consistent with a mechanism whereby EDTA causes detachment and
killing of biofilm cells.
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking
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| mogwaii 2006-03-22, 10:47 am |
| I've been fighting chronic strep for years. Many thanks on this very
important post.
| |
| David Wright 2006-03-22, 10:47 am |
| In article <1141752980.532820.231230@j33g2000cwa.googlegroups.com>,
mogwaii <borgersbrent@yahoo.com> wrote:
>I've been fighting chronic strep for years. Many thanks on this very
>important post.
That can't possibly be true. Surely a dose or two of silver would
have cured you completely.
-- David Wright :: alphabeta at prodigy.net
These are my opinions only, but they're almost always correct.
"If you can't say something nice, then sit next to me."
-- Alice Roosevelt Longworth
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| mogwaii 2006-03-22, 10:47 am |
|
David Wright wrote:
> In article <1141752980.532820.231230@j33g2000cwa.googlegroups.com>,
> mogwaii <borgersbrent@yahoo.com> wrote:
>
> That can't possibly be true. Surely a dose or two of silver would
> have cured you completely.
>
Goes to show you know crap about biofilms.
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| Mark Probert 2006-03-22, 10:47 am |
| mogwaii wrote:
> David Wright wrote:
>
> Goes to show you know crap about biofilms.
I thought that you were saying that silver was the miracle drug that
would cure all.
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| On 8 Mar 2006 06:41:08 -0800, "mogwaii" <borgersbrent@yahoo.com>
Huffed and Puffed the following into the madness of usenet:
>
>David Wright wrote:
>
>Goes to show you know crap about biofilms.
so exactly how did you use this info to cure your problem?
--
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President, or that we are to stand by the President
right or wrong, is not only unpatriotic and servile,
but is morally treasonable to the American public."
....Theodore Roosevelt
(o ô)
--ooO-(_)-Ooo--------------------
"I don't know half of you
half as well as I should like;
and I like less than half of you
half as well as you deserve."
....Bilbo Baggins
Jesus never hated anyone.
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| vernon 2006-03-22, 10:47 am |
|
"Mark Probert" <markprobert@lumbercartel.com> wrote in message
news:GhCPf.207$Uz7.8@fe09.lga...
> mogwaii wrote:
>
> I thought that you were saying that silver was the miracle drug that would
> cure all.
>
>
Only if there are little electrons skittering around here and there.
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| Mogwai fear Satan 2006-03-22, 10:47 am |
|
Mark Probert wrote:
> mogwaii wrote:
>
> I thought that you were saying that silver was the miracle drug that
> would cure all.
I should have been dead of a subdural empyema about 3-4 years ago, so
for me thats a miracle.In biofilms cells perform different functions,
it's an organism unto itself. Only the outer surface is active so I
have to kill this thing one cellular layer at a time. I have to kill it
faster than it can grow. Not an easy task. It'c common in catheters and
such. Traditional abx are worthless. The key they say is to break up
the communication between cells. Perhaps this is what EDTA is doing?
don't know but I'm gonna try it. I bought some EDTA suppositories. care
to help a guy out?
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<ironjustice@aol.com> wrote in message
His usual shite.
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| Mogwai fear Satan 2006-03-22, 10:47 am |
|
Beav wrote:
> <ironjustice@aol.com> wrote in message
>
> His usual shite.
Nooooooo.
I don't care what his other post are, this article (which is referenced
quite nicely) is of EXTREME importance to anyone with knowledge of
biofilms and the problems associated with them. Understand the article
before blasting it next time.
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| Simm Webb 2006-03-22, 10:47 am |
| Mogwai fear Satan wrote:
> Beav wrote:
>
>
>
> Nooooooo.
> I don't care what his other post are, this article (which is referenced
> quite nicely) is of EXTREME importance to anyone with knowledge of
> biofilms and the problems associated with them. Understand the article
> before blasting it next time.
>
Another braying shill.
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| Mogwai fear Satan 2006-03-22, 10:47 am |
|
Simm Webb wrote:
> Mogwai fear Satan wrote:
>
>
> Another braying shill.
What point, if any, are you trying to make? Are you stating biofilms
are NOT a problem. Stanford thinks otherwise.
Antibiotic resistance of biofilms
Bacterial biofilms are responsible for several chronic diseases that
are very difficult to treat. Examples are: cystic fibrosis,
endocarditis, cystitis, and infections caused by indwelling medical
devices. Biofilm bacteria show much greater resistance to antibiotics
than their free-living counterparts and our interest is to investigate
the mechanistic basis of this phenomenon. One potential reason for this
increased resistance is the penetration barrier that biofilms may
present to antimicrobials. ......
http://www.stanford.edu/~amatin/Mat...age/Biofilm.htm
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| Simm Webb 2006-03-22, 10:47 am |
| Mogwai fear Satan wrote:
> Simm Webb wrote:
>
>
>
> What point, if any, are you trying to make? Are you stating biofilms
> are NOT a problem. Stanford thinks otherwise.
>
>
Bray on you XXX.
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| Mogwai fear Satan 2006-03-22, 10:47 am |
| uh huh thats what I thought.
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| BJ in Texas 2006-03-22, 10:47 am |
| mogwaii <borgersbrent@yahoo.com> wrote:
|| I've been fighting chronic strep for years. Many thanks on
|| this very important post.
Mogwaii, leave this newsgroup!
Satan
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| Mogwai fear Satan 2006-03-22, 10:47 am |
|
BJ in Texas wrote:
> mogwaii <borgersbrent@yahoo.com> wrote:
> || I've been fighting chronic strep for years. Many thanks on
> || this very important post.
>
> Mogwaii, leave this newsgroup!
>
> Satan
Make me.
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"Mogwai fear Satan" <borgersbrent@yahoo.com> wrote in message
news:1141943276.709266.47610@i40g2000cwc.googlegroups.com...
>
> Beav wrote:
>
> Nooooooo.
> I don't care what his other post are, this article (which is referenced
> quite nicely) is of EXTREME bullshit.
Yeah, I know and I know youi're nothing more han a shill for the twat.
--
Beav
OMF#19
VN 750
Zed Thou
mail is beavis dot original at ntlworld dot com (with the obvious changes)
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"Mogwai fear Satan" <borgersbrent@yahoo.com> wrote in message
news:1142110673.296042.96630@e56g2000cwe.googlegroups.com...
> uh huh thats what I thought.
Well he does have a point.
--
Beav
OMF#19
VN 750
Zed Thou
mail is beavis dot original at ntlworld dot com (with the obvious changes)
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